RV
SF
lain
are
the
reen
ast
r to
‚the
sing
quality, these image processing functions can also be
used to solve a number of 2D image analysis
problems.
4.2.2 Orientations, 3D and 2D Surveying. Like
the conventional photogrammetric surveying procedure,
the operation of model surveying for EM images
follow the usual steps of relative orientation, absolute
orientation, and various surveying work.
At the relative orientation phase, only a part of the
left and the right EM images are displayed on the
screen at the same time(see Fig.5). In order to
measure the screen coordinates of any photo point,
the operator can not only move the two cursors on
the left and the right images arbitrarily but also
control the images panning and scrolling automatically.
The relative orientation is solved by least squares
using the Eqs. (6). Once the relative orientation is
completed, the left and the right images are rotated
respectively according to the 01 and 6 > calculated by
the relative orientation. The images used in later
surveying stage are these two rotated images.
Rotating the left and the right images means that the
relative orientation elements( 0 4 and 0 z)have been ”
put” into the left and the right images respectively.
So, to a given stereomodel, the relative orientation
needs to be done just one time for subsequent
surveying work.
Since the status of the specimen stage, during the
procedure of taking the left and the right
micrographs, can be controlled procisely, it is quite
easy to horizontalize the stereomodel after the relative
orientation. Usually, one is solely concerned with the
relative information about the specimen instead of the
absolute information, therefore, the offset in similarity
transformation is not needed. Only in the case of the
status of the specimen stage cann't be controlled
procisely, the formula of similarity transformation is
used in the absolute orientation with EM images.
After relative orientation and absolute orientation,
various 3D data can be acquired. All 3D data
processing functions have been listed in Fig.3.
Surveyiny DSM is the main task of EM images 3D
processing (volume, 3D area and perspective image all
use DSM). The system provides two ways for
surveying DSM. In most situation, automatic generate
DSM can be used. In this case, the stereomodel of
the specimen is scannered along the lines parallel to
the tilt axis of the specimen stage, and the mode
coordinates are computed at regular intervals on these
lines. Each new DSM point is marked on the screen
by a red point superimposed on its conjugate point,
this helps cheching and keeping track of the operation
as it proceeds. The best correlated point is determined
by the maximum value of the following equation:
cx as sb a 3)
k = ——— (7)
253,25 by
Where as 4 represents the grey value of pixel ij in the
window of the left image and bs, the grey value of
pixel ij in the window of the right image.
Some times, an error correlated point may be
calculated, in this case, the operator can interfere in
the procedure by putting the red point on the correct
conjugate point, then the automatic correlation go on.
4.2.3 Data, Graphic And Image Editing. In many
cases, the users want to edit a data file, graphics and
images. Certainly, the editing work can be done by
using some popular text and graphics editing
softwares. But this must lead to inconvenience in
operating. EM3DPS provides some functions for
editing data files, graphics and images. Although they
are not perfect, they can meet most of the
requirements.
4.2.4 Data Format Transformation. Electron
microscope users have various image input
instruments. To EM3DPS, the only difference of these
image input instruments is the data format of their
image files. In order to make EM3DPS surport as
many as possible image input instruments, the system
supplies a function of converting several types of
image files to EM3DPS(input to EM3DPS). In
adition, the cartographic plotters or printers owned by
electron microscope users are also manifold. There are
two methods to solve the problem of outputting the
cartographic information on various plotters or
printers:
e write various drawing drive programs
corresponding to each kind of plotter or printer.
® take advantage of the current popular
graphic softwares, since, usually, these softwares
surport many types of plotters or printers.
EM3DPS uses the second method and provides a
function of converting the graphic file of EM3DPS to
the *.dwg files of Auto CAD(EM3DPS to Auto
CAD).
4.2.5 Results outputting. The main aim of EM 3D
data acquiring is to analyse the microcosmic structure
of the specimen. So it is important to provides many
ways for outputting various 3D or 2D data. Besides
some conventional cartographic output functions, the
system also provides some special 3D or 2D output
functions. The perspective maps can only reflect the
microcosmic structure qualitatively. In order to display
the microcosmic structure qualitatively and
quatitatively, the system gives two functions: color
perspective map and stereo contour line(see Fig.3).
The former function means that the contour line
information are added into the DSM, and when the
DSM is displayed perspectively, different colors are
used between two contour lines. In order to reflect the
microcosmic structure of the specimen more audio-
visually, the system also provides a function of
displaying perspective image. This function needs a
DSM which includes the Z coordinate of each pixel.