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LABORATORY MEASUREMENTS ON HERBACEUS SPECIES 
GROWN IN CONTROLLED ATMOSPHERE BY MEANS OF 
THE ENEA LIDAR FLUOROSENSOR 
R.BARBINI, F.COLAO, R.FANTONI, R.GUARINI 1 , A.PALUCQ, S.RIBEZZO 
ENEA, Dipartimento Innovazione, Settore Elettroottica e Laser 
P.O. Box 65 
1-00044 Frascati RM (Italy) 
G.LORENZENI, N.PUCQ 
Dip. Coltivazione e Difesa Specie Legnose, Univ. Pisa 
1-56010 San Pietro a Grado - Pisa (Italy) 
M.LIPUCCI DI PAOLA 
Dip. Biologia Piante Agrarie, Univ. Pisa 
Viale delle Piagge, 23 
1-56124 Pisa (Italy) 
ABSTRACT 
The lidar fluorosensor apparatus built at the ENEA Research Centre in Frascati, has been proven to be a versa 
tile instrument for analyzing the Laser Induced Fluorescence (LIF) of chlorophyll both in the spectral and in the 
time domains. The system, has been applied in the spectral domain to study the effects of gaseous pollutants on 
heibaceus species. Seme preliminary results are hereafter presented and discussed. 
KEYWORDS: Fluorosensor, Remote Sensing, Pollutant, S0 2 , 0 3 . 
I. INTRODUCTION 
LIF lidar systems have been recently proven to be very useful instruments in remote monitoring of vegetation 
health, since they are capable to identify and measure the stress status of plants [1]. The lidar fluorosensor appa 
ratus built at the ENEA Centre in Frascati, has been used to monitor some herbaceus species grown under fully 
controlled conditions in the glasshouse of the Pisa University. Laboratory experiments have been carried out to 
check the effects of plant on poisoning with the most common pollutant gases, which can be found with high 
concentrations in urban and industrial areas. The purpose was to ascertain the remote sensing apparatus sensitiv 
ity to the effects of these pollutant gases and to assess the validity of the present analysis of LIF data. The plant 
samples have been divided in two groups for each species, the former containing the control samples, the latter 
containing samples polluted with S0 2 and 0 3 at concentration typically expected in the field. Effects of the pol 
lutant gases have been monitored on the vegetation pigments emission bands through the whole visible region. 
In particular, the F690/F735 ratio (red/red ratio) of chlorophyll fluorescence has been considered. Differences 
among the various groups of plants were found in several spectral bands and are here discussed. * 
* Student