i i i mul
10 2
a/b RATIO
ons of the emitters in
h chlorophyll a/b ratio.
> (Nipponbare and Norm
s (CMV-15, CMV-16,
88 ) were used. The data
mbols indicate • F680,
! A F745.
TH(nm)
tee spectra of rice wild-
lutant (MGS- 88 ) plant
(diode-laser) light,
-type plant(a) was 632
autant plant (b) 22.9
brane lipids and protein
induce dissociation of
m I (Fig. 2).
»served at 45°C were
I with exhaust gases of
ur oxides and nitrogen
contained in the exhaust
he reaction center of
& Sugahara, 1979),
f emissions from core
:ver, the chemicals seem
of photosystem II based
tion of 0 3 was different
mild fumigation of Oj
eripheral antenna of
3 incorporated into cells
are considered to generate large amounts of superoxide
radicals (Asada et al., 1977; Peiser & Yang, 1977) which
prevent functions of the protective mechanisms,
superoxide dismutase activity (McCord & Fridovich,
1969) and productions of ascorbate and reduced
glutathione (Asada et al., 1977), for naturally generated
superoxide radicals by photosystem I. Therefore, it
primarily acts on photosystem I. Another action of 0 3
on cell organizations is perturbation of membrane
structures through distortion of lipid bilayers.
Superoxide radicals produced from 0 3 cleave double
bonds of fatty acids rich in chloroplasts, resulting in
separation of peripheral antennas from photosystem □, as
well as photosystem I (Fig. 4c).
The assumed emitters in the current investigation do
not correspond to detailed structures of photosystems
and may involve other components than those assigned
above, however, the spectral changes due to high
temperature and air-pollutants, are primarily explained
by changes of emissions from the emitter components
which are sensitive to variable intrinsic and extrinsic
conditions of leaves. Measurements of laser induced
chlorophyll fluorescence and spectral analysis using the
simulated emitter peaks will provide information as to
how far light reactions of photosynthesis are affected
horn various environmental changes, which is useful for
monitoring terrestrial vegetations in remote sensing.
5. REFERENCES
Arnon, D. I. 1949. Copper enzymes in isolated
chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant
Physiology 24,1-15.
Asada, K., Takahashi, M., Tanaka, K. & Nakano, Y.
1977. Formation of active oxygen and its fate in
chloroplasts. In: Hayashi, O. and Asada, K. (eds)
Biochemical and Medical Aspects of Active Oxygen,
pp.45-63. University of Tokyo Press, Tokyo
Berry, J. & Bjorkman, O. 1980. Photosynthetic
response and adaptation to temperature in higher
plants. Annual Review of Plant Physiology 31, 491-
543.
Canaani, O. & Malkin, S. 1984. Distribution of light
excitation in an intact leaf between the two
photosystems of photosynthesis. Changes in absorption
cross-sections following state 1 - state 2 transitions.
Biochimica et Biophysica Acta 766,513-524.
Chappelle, E. W., Wood, Jr. F. M., McMurtrey III J.
E. & Newcomb, W. W. 1984. Laser-induced
fluorescence of green plants. 1: A technique for the
remote detection of plant stress and species
differentiation. Applied Optics 23,134-138.
Fork, D. C. & Satoh, K. 1986. The control by state
transitions of the distribution of excitation energy in
photosynthesis. Annual Review of Plant Physiology 37,
335- 361.
Goltsev, V., Yordanov, I., Stoyanova, T. & Popov, O.
1987. High temperature damage and acclimation of
the photosynthetic apparatus. Planta 170,478-488.
Holzwarth, A. R., Haehnel, W., Ratajczak, R.,
Bittersmann, E. & Schatz, G. H. 1990. Energy
transfer kinetics in photosystem I particles isolated
from Synechococcus sp. and from higher plants. In:
Current Research in Photosynthesis Vol. II pp.611-
614. Khiwer Academic Publishers.
Islam, K. 1987. The rate and extent of phosphorylation
of the two light-harvesting chlorophyll a/b binding
protein complex (LHC-II) polypeptides in isolated
spinach thylakoids. Biochimica et Biophysica Acta
893,333-341.
Kano, H. 1987. Photosynthetic unit size and the
efficiency of photoreactions in intact leaves of rice
chlorina mutants. Plant Physiology (Life Science
Advances) 6,259- 264.
Kano, H., Koizumi, M., Katsura, N. & Inada, K.
1988. Photochemical reactions of rice chlorina mutant,
CMV-44. Japanese Journal of Crop Science 57, 360-
365.
Koizumi, M., Kano, H., Katsura, N. & Inada, K.
1990. The effect of chlorophyll content on changes of
photochemical reactions in intact kidney bean leaves.
Plant, Cell and Environment 13,65-71.
Kocsanyi, L., Haitz, M. & Lichtenthaler, H. K. 1988.
Measurement of the laser-induced chlorophyll
fluorescence kinetics using a fast acoustooptic device.
In: Lichtenthaler, H. K. (ed) Applications of
Chlorophyll Fluorescence, pp.99-107. Kluwer
Academic Publishers, Dordrecht, Boston, London
Marchiarullo, M. A. & Ross, R. T. 1985. Resolution
of component spectra for spinach chloroplasts and
green algae by means of factor analysis. Biochimica et
Biophysica Acta 867,52-63.
McCord, J. M. & Fridovich, I. 1969. Superoxide
dismutase - An enzymic function for erythrocuprein
(hemocuprein). The Journal of Biological Chemistry
244,6049-6055.
Oquist, G. & Fork, D. C 1982. Effects of desiccation
on the 77°K fluorescence properties of the liverwort
Porella navicularis and the isolated lichen green alga
Trcbowda pyriformis. Physiologia Plantarum 56, 63-
68 .
Peiser, G. D. & Yang, S. F. 1977. Chlorophyll
destruction by the bisulfite-oxygen system. Plant
Physiology 66 ,277-281.
Rijgersberg, C. P., Amesz, J., Thielen, A. P. G. M. &.
Swager, J. A. 1979. Fluorescence emission spectra of
chloroplasts and subchloroplast preparations at low
temperature. Biochimica et Biophysica Acta 545, 473-
482.
Rinderle, U. & Lichtenthaler, H. K. 1988. The
chlorophyll fluorescence ratio F690/F735 as a possible
stress indicator. In: Lichtenthaler, H. K. (ed)
Applications of Chlorophyll Fluorescence, pp.189-
196. Kluwer Academic Publishers, Dordrecht,
Boston, London
Schreiber, U. & Armond, P. A 1978. Heat-induced
changes of chlorophyll fluorescence in isolated
chloroplasts and related heat-damage at the pigment
level. Biochimica et Biophysica Acta 562,138-151.